EC Number: 2.5.1.15 Protein ID:9055-61-2 Organism: Salmonellaa enteritidis Etiological risk group: N/A Gene Ontology: GO:0004156DefinitionCatalysis of the reaction: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate Disease Information:Salmonella enteritidis is responsible for fever, abdominal cramps, and diarrhea. It is common in food poisoning and causes a typhoid-like disease in mice. It is transmitted through contaminated water, contact with others, and through food. There is no cure for these strains of Salmonella as they eventually leave the body. However, mutant strains could lead to epidemics such as the recent outbreak of salmonella through consumption of peanut butter. Widespread contamination can cause problems for several groups of people and could result in recall of products.
Essentiality of Target: ExPasy link: Expasy
This enzyme dihydropteroate in bacteria and is essential to the folate biosynthesis pathway. Cofactor production is essential for metabolism. diphosporylase is vital for catalyzing the condensation of 6-hydroxymethyl-7,8-dihydropteridine pyrophosphate to para-aminobenzoic acid to form 7,8-dihydropteroate.
Substrates not readily available - Dr. B 060617
EC Number: 2.5.1.15
Protein ID: 9055-61-2
Organism: Salmonellaa enteritidis
Etiological risk group: N/A
Gene Ontology:
GO:0004156DefinitionCatalysis of the reaction: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate
Disease Information:Salmonella enteritidis is responsible for fever, abdominal cramps, and diarrhea. It is common in food poisoning and causes a typhoid-like disease in mice. It is transmitted through contaminated water, contact with others, and through food. There is no cure for these strains of Salmonella as they eventually leave the body. However, mutant strains could lead to epidemics such as the recent outbreak of salmonella through consumption of peanut butter. Widespread contamination can cause problems for several groups of people and could result in recall of products.
Links:
Science Direct Article
CDC
Medicine Net
Link to TDR Targets: Not present
Essentiality of Target: ExPasy link: Expasy
This enzyme dihydropteroate in bacteria and is essential to the folate biosynthesis pathway. Cofactor production is essential for metabolism. diphosporylase is vital for catalyzing the condensation of 6-hydroxymethyl-7,8-dihydropteridine pyrophosphate to para-aminobenzoic acid to form 7,8-dihydropteroate.
Druggable Target: http://pubs.acs.org/doi/abs/10.1021/acschembio.5b00490
Link to BRENDA: BRENDA EC Number and Enzyme Information
Enzyme Assay: Label-free Fluorescence
3D Structure: Link to PDB (vibrio fisheri synonymous structure)
Amino Acid Length: 279
Extinction Coefficient: Expasy
Molecular Weight: Expasy
Amino Acid Sequence: UniPro
10 20 30 40 50
MVTVFGILNL TEDSFFDESR RLDPAGAVTA AIEMLRVGSD VVDVGPAASH
60 70 80 90 100
PDARPVSPAD EIRRIAPLLD ALSDQMHRVS IDSFQPETQR YALKRGVGYL
110 120 130 140 150
NDIQGFPDPA LYPDIAEADC RLVVMHSAQR DGIATRTGHL RPEDALDEIV
160 170 180 190 200
RFFEARVSAL RRSGVAADRL ILDPGMGFFL SPAPETSLHV LSNLQKLKSA
210 220 230 240 250
LGLPLLVSVS RKSFLGATVG LPVKDLGPAS LAAELHAIGN GADYVRTHAP
260 270
GDLRSAITFS ETLAKFRSRD ARDRGLDHA
Pairwaise BLASTP in NCBI: Link to BLASTP
Query Coverage: 99%
Max % Identities: 99%
% Positives: 99%
Chain used for homology: A
TMpred Graph : Output graph
Inhibitor: Sulfadizine
CDS Gene Sequence: Sequence Information
GC% Content for gene: 6.776895%
CDS Gene Sequence (codon optimized) - N/a
GC% Content for gene (codon optimized): N/a